Device
Part:BBa_K1903013:Design
Designed by: Sofía Vieto Fonseca Group: iGEM16_TEC-Costa_Rica (2016-10-14)
Plac-RBS-Split TEV-IntN DnaE + PA1-RBS + Split TEV-IntC DnaE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1044
Illegal NheI site found at 1067 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 544
Illegal SapI.rc site found at 1209
Illegal SapI.rc site found at 1519
Design Notes
For this BioBrick we fused two devices BBa_K1903006 and BBa_K1903008. This is a system that will react Inteins from DnaE to trigger the reconstitution of the TEV, under the presence of IPTG to induce the BBa_K1903006 BioBrick.